Bin1 is a conserved member of the BAR family of genes that have been implicated in diverse cellular processes including endocytosis, actin organization, programmed cell death, stress responses, and transcriptional control. The first mammalian BAR protein to be discovered, Amphiphysin I (AmphI), was identified in an immunoscreen for proteins associated with the plasma membranes of synaptic neurons (1), functions in the control of clathrin-dependent synaptic vesicle endocytosis. The mammalian Bin1 gene was first identified in a two hybrid screen for polypeptides that bind to the N-terminal Myc box 1 (MB1) portion of the c-Myc oncoprotein (2). Bin1 is similar to AmphI in overall structure, with an N-terminal BAR domain and a C-terminal SH3 domain (2). However, the Bin1 gene is more complex than the AmphI gene, encoding at least seven different splice variants that differ widely in subcellular localization, tissue distribution, and ascribed functions (3, 4, 5, and 6); Alternate splicing of the Bin1 gene results in ten transcript variants encoding different isoforms (7). Bin1 is expressed ubiquitously in mammalian cells. Certain splice variants of Bin1 are expressed in the neurons, muscle cells or tumor cells. Bin1 may act as a tumor suppressor and inhibits malignant cell transformation (7). Studies in mouse suggest that this gene plays an important role in cardiac muscle development (8). Bin1 has also been implicated in Alzheimer's disease and cardiac disease (9, 10). Defects in Bin1 are the cause of centronuclear myopathy autosomal recessive (ARCNM) [MIM: 255200]; also known as autosomal recessive myotubular myopathy (11).
Monoclonal antibodies available from LDI include splice-isoform specific antibodies 99D and 12A, for generally expressed or brain-specific isoforms, respectively; and the pan-Bin1 antibody 2F11, which recognizes all Bin1 isoforms.
- Lichte, B., R. et al. (1992) EMBO J. 11:2521-30.
- Sakamuro, D. et al. (1996) Nat. Genet. 14:69-77.
- Butler, M. H. J. et al. (1997) Cell Biol. 137:1355-67.
- Ramjaun, A. R. et al. (1997) J. Biol. Chem. 272:16700-6.
- Tsutsui, K. et al. (1997) Biochem. Biophys. Res. Commun. 236:178-83.
- Wechsler-Reya, R., D. et al. (1997) J. Biol. Chem. 272:31453-8.
- Prendergast G.C. et al. (2009) Biochim Biophys Acta.1795:25-36.
- Muller A. J. et al. (2003) Mol Cell Biol. 23:4295-306.
- Joseph H. L. et al. (2011) Arch Neurol. 69: 320-8.
- Hong T et al. (2010) PloS biology 8:e1000312.
- Nicot A.-S. et al. (2007) Nat. Genet. 39:1134-9.
Anti-Bin1, Clone 99D (LDI-301)
Antigen Information
Recombinant fragment corresponding to amino acids 189-398 of human Bin1
Source Antibody/Purification Information
Mouse monoclonal [99D] to Bin1, isotype IgG
Protein G column purified, 1 mg/ml
Species Cross Reactivity
Mouse, rat, human
Applications
Western, ELISA, IP, IHC (acetone-fixed frozen sections), FACS analysis
Recommended Dilutions for Each Application
Western - 1:1000
Immunoprecipitation - use at an assay dependent dilution
Immunohistochemistry - use at an assay dependent dilution
Anti-Bin1, Clone 2F11 (LDI-302)
Antigen Information
GST chimeric protein that encoded human Bin1 BAR domain
Source Antibody/Purification Information
Mouse monoclonal [2F11] to Bin1, isotype IgG1
Protein G column purified, 1mg/ml
Species Cross Reactivity
Mouse, human
Applications
Western, IP, IHC (formalin-fixed paraffin-embedded sections), ELISA
Recommended Dilutions for Each Application
Western - 1:1000
Immunoprecipitation - use at an assay dependent dilution
Immunohistochemistry - use at an assay dependent dilution
Anti-Bin1, Clone 12A (LDI-303)
Antigen Information
GST-Bin1 12A exon
Source Antibody/Purification Information
Mouse monoclonal [1810] to Bin1, isotype IgG
Species Cross Reactivity
Human
Applications
Western, ELISA
Recommended Dilutions for Each Application
Western - use at an assay dependent dilution
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