Bin3 is a Bin1-related gene discovered by LIMR researchers which encodes an evolutionarily conserved and ubiquitously expressed member of the BAR superfamily, curved membrane and GTPase-binding proteins implicated in signal transduction and vesicular trafficking (1). Research has identified roles for Bin3 in cell division and cancer, where it acts as a tumor suppressor. Bin3 is found in all cells with additional specific isoforms found in the immune system and in the brain.

In humans, Bin3 maps to chromosome 8p21.3, a region widely implicated in cancer suppression that is often deleted in non-Hodgkin's lymphomas and various epithelial tumors (2). A LIMR study in a mouse model suggests that homozygous inactivation of Bin3 causes cataracts and an increased susceptibility to lymphomas during aging (3).

Antigen Information

Recombinant full length human Bin3 with a COOH-terminal glutathione S-transferase (GST) fusion tag

Source Antibody/Purification Information

Mouse monoclonal 3A4, isotype IgG2b

Species Cross Reactivity

Human, mouse; others not tested

Applications

Western, immunoprecipitation, ELISA

Recommended Dilutions for Each Application

Western - use at an assay dependent dilution
Immunoprecipitation - use at an assay dependent dilution

1. Routhier EL, Burn TC, Abbaszade I, Summers M, Albright CF, Prendergast GC (2001). Human Bin3 complements the F-actin localization defects caused by loss of Hob3p, the fission yeast homolog of Rvs161p. J. Biol. Chem. 276(24):21670-7.


2. Birnbaum D, Adelaide J, Popovici C, Charafe-Jauffret E, Mozziconacci MJ, Chaffanet M (2003). Chromosome arm 8p and cancer: a fragile hypothesis. Lancet Oncol. 4(10):639-642.


3. Ramalingam A, Duhadaway JB, Sutanto-Ward E, Wang Y, Dinchuk J, Huang M, Donover PS, Boulden J, McNally LM, Soler AP, Muller AJ, Duncan MK, Prendergast GC (2008). Bin3 deletion causes cataracts and increased susceptibility to lymphoma during aging. Cancer Res. 68(6):1683-90.

To Place an Order

By Phone: 484-476-3474
By Fax: 484-476-8533
By Email: sales@limrdev.com

By Mail:
LIMR Development, Inc.
Sales Department
Attn: J. Wynne
100 East Lancaster Avenue
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