Indoleamine 2,3-dioxygenase 1 (IDO1) is a 41-42 kD intracellular enzyme that regulates the immune system by catabolizing tryptophan into kynurenine (1). IDO1 modulates levels of the amino acid tryptophan, which is vital for cell growth, but is also involved in the suppression of the immune response (2). IDO1 effects on immune suppression are due to decreased tryptophan availability and the generation of tryptophan metabolites, resulting in negative effects on T lymphocytes, including proliferation, function and survival (3, 4). IDO1 may be involved in the suppression of the immune response to tumors, and blocking the IDO1 pathway may be a potential target for immunotherapy (5, 6, 7). IDO1 is expressed in a wide variety of tissues and can be upregulated by interferon gamma and other inflammatory cytokines (8). IDO is involved in cancer, chronic viral disease, autoimmune disease, allergy and other immune disorders. Emerging evidence also suggests a role for IDO in modulating inflammation, Alzheimer's disease, infectious disease, as well as blood pressure and lipid management.

Antigen Information

Amino acids 78-184 of human IDO1 fused to GST

Source Antibody/Purification Information

Mouse monoclonal 10.1 to IDO1, isotype IgG3
Protein G column purified

Species Cross Reactivity

Mouse, human

Applications

Western, IP, IHC (formalin-fixed paraffin-embedded sections), ELISA

Recommended Dilutions for Each Application

Western - use at an assay dependent dilution
Immunoprecipitation - use at an assay dependent dilution
Immunohistochemistry - use at an assay dependent dilution

1. Mellor AL, Munn DH. IDO expression by dendritic cells: tolerance and tryptophan catabolism. Nat. Rev. Immunol. 2004;4 (10): 762-74.
2. Godin-ethier J, Hanafi LA, Piccirillo CA et-al. Indoleamine 2,3-dioxygenase expression in human cancers: clinical and immunologic perspectives. Clin. Cancer Res. 2011;17 (22): 6985-91.
3. Mellor AL, Keskin DB, Johnson T et-al. Cells expressing indoleamine 2,3-dioxygenase inhibit T cell responses. J. Immunol. 2002;168 (8): 3771-6.
4. Frumento G, Rotondo R, Tonetti M et-al. Tryptophan-derived catabolites are responsible for inhibition of T and natural killer cell proliferation induced by indoleamine 2,3-dioxygenase. J. Exp. Med. 2002;196 (4): 459-68.
5. Muller AJ, Duhadaway JB, Donover PS et-al. Inhibition of indoleamine 2,3-dioxygenase, an immunoregulatory target of the cancer suppression gene Bin1, potentiates cancer chemotherapy. Nat. Med. 2005;11 (3): 312-9.
6. Löb S, Königsrainer A, Rammensee HG et-al. Inhibitors of indoleamine-2,3-dioxygenase for cancer therapy: can we see the wood for the trees? Nat. Rev. Cancer. 2009;9 (6): 445-52.
7. Uyttenhove C, Pilotte L, Théate I et-al. Evidence for a tumoral immune resistance mechanism based on tryptophan degradation by indoleamine 2,3-dioxygenase. Nat. Med. 2003;9 (10): 1269-74.
8. Däubener W, Mackenzie CR. IFN-gamma activated indoleamine 2,3-dioxygenase activity in human cells is an antiparasitic and an antibacterial effector mechanism. Adv. Exp. Med. Biol. 1999;467 : 517-24.

To Place an Order

By Phone: 484-476-3474
By Fax: 484-476-8533
By Email: sales@limrdev.com

By Mail:
LIMR Development, Inc.
Sales Department
Attn: J. Wynne
100 East Lancaster Avenue
Wynnewood, PA 19096

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